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Article

Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA, and actin filaments.

Proceedings of the National Academy of Sciences of the United States of America - 11 Sept 2018

Espinoza-Sanchez Sofia, Metskas Lauren Ann, Chou Steven Z, Rhoades Elizabeth, Pollard Thomas D

Abstract excerpt

We used fluorescence spectroscopy and EM to determine how binding of ATP, nucleation-promoting factors, actin monomers, and actin filaments changes the conformation of Arp2/3 complex during the process that nucleates an actin filament branch. We mutated subunits of Schizosaccharomyces pombe Arp2/3 complex for labeling with fluorescent dyes at either the C termini of Arp2 and Arp3 or ArpC1 and ArpC3. We measured...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 30150414
DOI: 10.1073/pnas.1717594115
PMCID: PMC6140485

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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