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Rational design of a highly reactive dicysteine peptide tag for fluorogenic protein labelling.

Organic & biomolecular chemistry - 29 Aug 2018

Strmiskova Miroslava, Tsao Kelvin, Keillor Jeffrey W

Abstract excerpt

Rationally designed libraries of a short helical peptide sequence containing two cysteine residues were screened kinetically for their reactivity towards complementary dimaleimide fluorogens. This screening revealed variant sequences whose reactivity has been increased by an order of magnitude relative to the original sequence. The most reactive engineered sequences feature mutant residues bearing positive...

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PMID: 30131994
DOI: 10.1039/c8ob01417e

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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