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Article

A bidirectional system for the dynamic small molecule control of intracellular fusion proteins.

ACS chemical biology - 18 Oct 2013

Neklesa Taavi K, Noblin Devin J, Kuzin Alexander P, Lew Scott, Seetharaman Jayaraman, Acton Thomas B, Kornhaber Gregory J, Xiao Rong, Montelione Gaetano Thomas, Tong Liang, Crews Craig M

Abstract excerpt

Small molecule control of intracellular protein levels allows temporal and dose-dependent regulation of protein function. Recently, we developed a method to degrade proteins fused to a mutant dehalogenase (HaloTag2) using small molecule hydrophobic tags (HyTs). Here, we introduce a complementary method to stabilize the same HaloTag2 fusion proteins, resulting in a unified system allowing bidirectional control of...

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Identifiers

PMID: 23978068
DOI: 10.1021/cb400569k
PMCID: PMC4113957

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