Article
Developmental barcoding of whole mouse via homing CRISPR.
Science (New York, N.Y.) - 31 Aug 2018
Kalhor Reza, Kalhor Kian, Mejia Leo, Leeper Kathleen, Graveline Amanda, Mali Prashant, Church George M
Abstract excerpt
In vivo barcoding using nuclease-induced mutations is a powerful approach for recording biological information, including developmental lineages; however, its application in mammalian systems has been limited. We present in vivo barcoding in the mouse with multiple homing guide RNAs that each generate hundreds of mutant alleles and combine to produce an exponential diversity of barcodes. Activation upon...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.