Article
Mechanistic effect of the human GJB6 gene and its mutations in HaCaT cell proliferation and apoptosis.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas - 23 Jul 2018
Lu Yuting, Zhang Ruili, Wang Zhenying, Zhou Shuhua, Song Yali, Chen Lamei, Chen Nan, Liu Wenmin, Ji Canan, Wu Wangli, Zhang Li
Abstract excerpt
We constructed lentiviral vectors containing the human wild-type GJB6 gene and the mutant variants A88V and G11R. The three proteins were stably expressed by the Tet-on system in the HaCaT cell line and used to study the functional effect of the variants. The CCK-8 assay and flow cytometric analyses were used to determine the levels of cell proliferation and apoptosis. Western blot analyses were performed to...
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