Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 Regulation in Naive Pluripotency Maintenance and Dissolution.

Cell reports - 10 Jul 2018

Li Meng, Yu Jason S L, Tilgner Katarzyna, Ong Swee Hoe, Koike-Yusa Hiroko, Yusa Kosuke

Abstract excerpt

The genetic basis of naive pluripotency maintenance and loss is a central question in embryonic stem cell biology. Here, we deploy CRISPR-knockout-based screens in mouse embryonic stem cells to interrogate this question through a genome-wide, non-biased approach using the Rex1GFP reporter as a phenotypic readout. This highly sensitive and efficient method identified genes in diverse biological processes and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Self Renewal
Share to Clustered Regularly Interspaced Short Palindromic Repeats

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Knockout Techniques

Share to Glycogen Synthase Kinase 3

Share to Mechanistic Target of Rapamycin Complex 1

Share to Mouse Embryonic Stem Cells

Share to Pluripotent Stem Cells

Share to Transcriptome

Source

Open in PubMed Open full text

Identifiers

PMID: 29996108
DOI: 10.1016/j.celrep.2018.06.027
PMCID: PMC6057492

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 Regulation in Naive Pluripotency Maintenance and Dissolution.PMID 29996108Read ↗

ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiationSemantic proximity Read ↗

Pten-mediated Gsk3β modulates the naïve pluripotency maintenance in embryonic stem cells.Semantic proximity Read ↗

An Elaborate Regulation of Mammalian Target of Rapamycin Activity Is Required for Somatic Cell Reprogramming Induced by Defined Transcription FactorsSemantic proximity Read ↗

Cooperative genetic networks drive a mammalian cell state transitionSemantic proximity Read ↗

Genetic exploration of the exit from self-renewal using haploid embryonic stem cells.Semantic proximity Read ↗

Controlling embryonic stem cell proliferation and pluripotency: the role of PI3K- and GSK-3-dependent signallingSemantic proximity Read ↗

Negative feedback via RSK modulates Erk-dependent progression from naïve pluripotency.Semantic proximity Read ↗

Transient Activation of Autophagy via Sox2-Mediated Suppression of mTOR Is an Important Early Step in Reprogramming to PluripotencySemantic proximity Read ↗

JAK/STAT3 regulated global gene expression dynamics during late-stage reprogramming processSemantic proximity Read ↗

ZZZ3 protects human embryonic stem cells from nucleolar stress by boosting mTOR/ribosome pathwaySemantic proximity Read ↗

Mitochondrial E3 ligase March5 maintains stemness of mouse ES cells via suppression of ERK signallingSemantic proximity Read ↗

TGFβ signalling is required to maintain pluripotency of human naïve pluripotent stem cellsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.