Article
Identification and Quantification of Heterogeneously-methylated DNA Fragments Using Epiallele-sensitive Droplet Digital Polymerase Chain Reaction (EAST-ddPCR).
Cancer genomics & proteomics - 1 Jan 2000
Menschikowski Mario, Jandeck Carsten, Friedemann Markus, Richter Susan, Thiem Dana, Lange Björn Sönke, Suttorp Meinolf
Abstract excerpt
BACKGROUND/AIM: DNA methylation plays an important role in the initiation and propagation of carcinogenesis; however, the role of heterogeneously methylated epialleles is currently not well studied, also due to the lack of sensitive, unbiased and high throughput methods. Here, a newly developed droplet digital PCR (ddPCR)-based method was evaluated regarding its ability to quantify such heterogeneously methylated...
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