Article
[Determinant of resistance to kanamycin in Streptomycin rimosus: amplification in the chromosome and reversible genetic instability].
Molekuliarnaia biologiia - 1 Jan 2000
Potekhin Ia A, Danilenko V N
Abstract excerpt
The study on the kanamycin resistance determinant (Kanr) in an oxytetracycline--producing strain of S. rimosus showed that it was capable of amplifying in the chromosome during selection for increasing the antibiotic resistance level. The amplification of the DNA fragment with a molecular weight of 10.3 MDa containing Kanr amounted to 300 copies per genome, which resulted in a more than 1000-fold increase in...
Topics
- Chromosome Mapping
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Restriction Enzymes
- DNA, Bacterial
- Drug Resistance, Microbial
- Electrophoresis, Polyacrylamide Gel
- Gene Amplification
- Kanamycin
- Phenotype