Article
[Isolation and study of the properties of Streptomyces kanamyceticus mutants with disorders of kanamycin biosynthesis].
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic] - 1 Jan 1995
Golets L M, Basiliia L I, Fedorenko V A
Abstract excerpt
The strains of Streptomyces kanamyceticus 1375 and 1 were exposed to N-nitroso-N-methyl urea, ethidium bromide and acriflavine and mutants with impaired biosynthesis of kanamycin (Kan-) were isolated. The majority of the mutants were genetically unstable and segregated with the formation of both the Kan- and Kan+ clones. The stable Kan- mutants were tested in pairs for the cosynthesis. The capacity of the mutants...
Topics
- Cloning, Molecular
- Culture Media
- Gene Expression Regulation, Bacterial
- Genetic Markers
- Kanamycin
- Mutation
- Streptomyces