Article
One-Pot Enzymatic Synthesis of D-Arylalanines Using Phenylalanine Ammonia Lyase and L-Amino Acid Deaminase.
Applied biochemistry and biotechnology - 1 Jan 2019
Zhu Longbao, Feng Guoqiang, Ge Fei, Song Ping, Wang Taotao, Liu Yi, Tao Yugui, Zhou Zhemin
Abstract excerpt
The phenylalanine ammonia-lyase (AvPAL) from Anabaena variabilis catalyzes the amination of substituent trans-cinnamic acid (t-CA) to produce racemic D,L-enantiomer arylalanine mixture owing to its low stereoselectivity. To produce high optically pure D-arylalanine, a modified AvPAL with high D-selectivity is expected. Based on the analyses of catalytic mechanism and structure, the Asn347 residue in the active...
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