Article
One step synthesis of unnatural β-arylalanines using mutant phenylalanine aminomutase from Taxus chinensis with high β-regioselectivity.
Enzyme and microbial technology - 1 Jul 2018
Zhu Longbao, Ge Fei, Li Wanzhen, Song Ping, Tang Hongjin, Tao Yugui, Liu Yi, Du Guocheng
Abstract excerpt
Phenylalanine aminomutase (TcPAM) from Taxus chinensis catalyzes the regioselective hydroamination of trans-cinnamic acid (t-CA) to yield β-phe. However, the final product mixture consists of both α- and β-phe owing to low regioselectivity, which is still a challenge to synthesize highly pure β-phe. Therefore, a modified TcPAM with high β-selectivity is expected. Based on the catalytic mechanism and structure,...
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