Article
Characterization of DCL4 missense alleles provides insights into its ability to process distinct classes of dsRNA substrates.
The Plant journal : for cell and molecular biology - 1 Jul 2018
Montavon Thomas, Kwon Yerim, Zimmermann Aude, Hammann Philippe, Vincent Timothée, Cognat Valérie, Bergdoll Marc, Michel Fabrice, Dunoyer Patrice
Abstract excerpt
In the model plant Arabidopsis thaliana, four Dicer-like proteins (DCL1-4) mediate the production of various classes of small RNAs (sRNAs). Among these four proteins, DCL4 is by far the most versatile RNaseIII-like enzyme, and previously identified dcl4 missense alleles were shown to uncouple the production of the various classes of DCL4-dependent sRNAs. Yet little is known about the molecular mechanism behind...
Topics
- Alleles
- Arabidopsis
- Arabidopsis Proteins
- Mutation, Missense
- RNA
- RNA, Double-Stranded
- Ribonuclease III
- Substrate Specificity