Article
The helicase and RNaseIIIa domains of Arabidopsis Dicer-Like1 modulate catalytic parameters during microRNA biogenesis.
Plant physiology - 1 Jun 2012
Liu Chenggang, Axtell Michael J, Fedoroff Nina V
Abstract excerpt
Dicer-Like1 (DCL1), an RNaseIII endonuclease, and Hyponastic Leaves1 (HYL1), a double-stranded RNA-binding protein, are core components of the plant microRNA (miRNA) biogenesis machinery. hyl1 null mutants accumulate low levels of miRNAs and display pleiotropic developmental phenotypes. We report the identification of five new hyl1 suppressor mutants, all of which are alleles of DCL1. These new alleles affect...
Topics
- Adenosine Triphosphate
- Alleles
- Amino Acid Sequence
- Arabidopsis
- Arabidopsis Proteins
- Base Sequence
- Catalytic Domain
- Cell Cycle Proteins
- Cloning, Molecular
- Enzyme Activation
- Genetic Complementation Test