Article
Primed CRISPR adaptation in Escherichia coli cells does not depend on conformational changes in the Cascade effector complex detected in Vitro.
Nucleic acids research - 4 May 2018
Krivoy Andrey, Rutkauskas Marius, Kuznedelov Konstantin, Musharova Olga, Rouillon Christophe, Severinov Konstantin, Seidel Ralf
Abstract excerpt
In type I CRISPR-Cas systems, primed adaptation of new spacers into CRISPR arrays occurs when the effector Cascade-crRNA complex recognizes imperfectly matched targets that are not subject to efficient CRISPR interference. Thus, primed adaptation allows cells to acquire additional protection against mobile genetic elements that managed to escape interference. Biochemical and biophysical studies suggested that...
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