Article
Construction of a New Phage Integration Vector pFIV-Val for Use in Different Francisella Species.
Frontiers in cellular and infection microbiology - 1 Jan 2018
Tlapák Hana, Köppen Kristin, Rydzewski Kerstin, Grunow Roland, Heuner Klaus
Abstract excerpt
We recently identified and described a putative prophage on the genomic island FhaGI-1 located within the genome of Francisella hispaniensis AS02-814 (F. tularensis subsp. novicida-like 3523). In this study, we constructed two variants of a Francisella phage integration vector, called pFIV1-Val and pFIV2-Val (Francisella Integration Vector-tRNAVal-specific), using the attL/R-sites and the site-specific integrase...
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