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Article

Mutations in the active site of Escherichia coli phosphofructokinase.

Nature - 1 Jan 2000

Hellinga H W, Evans P R

Abstract excerpt

The enzyme-catalysed transfer of a phosphoryl group from ATP is an important reaction in a wide variety of biological processes. We demonstrate here the essential function of an aspartate group in the catalysis of phosphoryl transfer by Escherichia coli phosphofructokinase, and the minor role of an arginine residue. We have used oligonucleotide-directed mutagenesis to replace two amino-acid residues which X-ray...

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PMID: 2953977
DOI: 10.1038/327437a0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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