Article
Conversion of allosteric inhibition to activation in phosphofructokinase by protein engineering.
Nature - 1 Jan 2000
Lau F T, Fersht A R
Abstract excerpt
Many enzymes are subject to allosteric control, often with inhibitors and activators binding to the same effector site. Phosphofructokinase in Escherichia coli is such an enzyme, being inhibited by phosphoenolpyruvate (PEP) and activated by ADP and GDP. How do individual interactions with effectors affect the balance between activation and inhibition, especially when both ligands share aspects of the same binding...
Topics
- Adenosine Diphosphate
- Chemical Phenomena
- Chemistry, Physical
- Dose-Response Relationship, Drug
- Enzyme Activation
- Enzyme Inhibitors
- Escherichia coli
- Guanosine Diphosphate
- Kinetics
- Mutation
- Phosphoenolpyruvate