Article
Probing the role of arginines and histidines in the catalytic function and activation of yeast 3-phosphoglycerate kinase by site-directed mutagenesis.
The Journal of biological chemistry - 25 Jun 1990
Sherman M A, Szpikowska B K, Dean S A, Mathiowetz A M, McQueen N L, Mas M T
Abstract excerpt
A cluster of conserved histidines and arginines (His-62, His-167, Arg-21, Arg-38, and Arg-168) in 3-phosphoglycerate kinase (PGK) has been implicated as possibly involved in the binding of 3-phosphoglycerate (3-PG) and/or stabilization of the negatively charged transition state. The role of these...
Topics
- Amino Acid Sequence
- Arginine
- Base Sequence
- Binding Sites
- Enzyme Activation
- Histidine
- Kinetics
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Phosphoglycerate Kinase
- Protein Conformation