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Article

Mycobacterium tuberculosis complex genotypes circulating in Nigeria based on spoligotyping obtained from Ziehl-Neelsen stained slides extracted DNA.

PLoS neglected tropical diseases - 1 Feb 2018

Molina-Moya Barbara, Gomgnimbou Michel K, Spinasse Lizania, Obasanya Joshua, Oladimeji Olanrewaju, Dacombe Russell, Edwards Thomas, Daragon Xavier-Olessa, Lawson Lovett, Abdurrahman Saddiq T, Cuevas Luis E, Dominguez Jose, Sola Christophe

Abstract excerpt

METHODS: All State TB control programmes in Nigeria were requested to submit 25-50 smear-positive Ziehl-Neelsen (ZN) stained slides for screening during 2013-2014. DNA was extracted from 929 slides for spoligotyping and drug-resistance analysis using microbead-based flow-cytometry suspension arrays. RESULTS: Spoligotyping results were obtained for 549 (59.1%) of 929 samples. Lineage 4 Cameroon sublineage (L4.6.2)...

Topics

Computational Biology
DNA, Bacterial
Genetic Variation
Genotype
Humans
Molecular Epidemiology
Molecular Typing
Mycobacterium tuberculosis
Nigeria

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phylogeography

Sputum

Staining and Labeling

Tuberculosis

Source

Open in PubMed Open full text

Identifiers

PMID: 29447161
DOI: 10.1371/journal.pntd.0006242
PMCID: PMC5831734

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-29447161-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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