Article
High-Throughput Quantification of Bacterial-Cell Interactions Using Virtual Colony Counts.
Frontiers in cellular and infection microbiology - 1 Jan 2018
Hoffmann Stefanie, Walter Steffi, Blume Anne-Kathrin, Fuchs Stephan, Schmidt Christiane, Scholz Annemarie, Gerlach Roman G
Abstract excerpt
The quantification of bacteria in cell culture infection models is of paramount importance for the characterization of host-pathogen interactions and pathogenicity factors involved. The standard to enumerate bacteria in these assays is plating of a dilution series on solid agar and counting of the resulting colony forming units (CFU). In contrast, the virtual colony count (VCC) method is a high-throughput...
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