Article
Achieving Plant CRISPR Targeting that Limits Off-Target Effects.
The plant genome - 1 Nov 2016
Wolt Jeffrey D, Wang Kan, Sashital Dipali, Lawrence-Dill Carolyn J
Abstract excerpt
The CRISPR-Cas9 system (clustered regularly interspaced short palindromic repeats with associated Cas9 protein) has been used to generate targeted changes for direct modification of endogenous genes in an increasing number of plant species; but development of plant genome editing has not yet fully considered potential off-target mismatches that may lead to unintended changes within the genome. Assessing the...
Topics
- CRISPR-Cas Systems
- Gene Editing
- Genome, Plant
- Mutation