Article
A posttranslational modification of the mitotic kinesin Eg5 that enhances its mechanochemical coupling and alters its mitotic function.
Proceedings of the National Academy of Sciences of the United States of America - 20 Feb 2018
Muretta Joseph M, Reddy Babu J N, Scarabelli Guido, Thompson Alex F, Jariwala Shashank, Major Jennifer, Venere Monica, Rich Jeremy N, Willard Belinda, Thomas David D, Stumpff Jason, Grant Barry J, Gross Steven P, Rosenfeld Steven S
Abstract excerpt
Numerous posttranslational modifications have been described in kinesins, but their consequences on motor mechanics are largely unknown. We investigated one of these-acetylation of lysine 146 in Eg5-by creating an acetylation mimetic lysine to glutamine substitution (K146Q). Lysine 146 is located in the α2 helix of the motor domain, where it makes an ionic bond with aspartate 91 on the neighboring α1 helix....
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