Article
A seminested PCR assay for detection and typing of human papillomavirus based on E1 gene sequences.
Diagnostic microbiology and infectious disease - 1 May 2018
Cavalcante Gustavo Henrique O, de Araújo Josélio M G, Fernandes José Veríssimo, Lanza Daniel C F
Abstract excerpt
HPV infection is considered one of the leading causes of cervical cancer in the world. To date, more than 180 types of HPV have been described and viral typing is critical for defining the prognosis of cancer. In this work, a seminested PCR which allow fast and inexpensively detection and typing of HPV is presented. The system is based on the amplification of a variable length region within the viral gene E1,...
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