Article
A real-time PCR approach based on SPF10 primers and the INNO-LiPA HPV Genotyping Extra assay for the detection and typing of human papillomavirus.
Journal of virological methods - 1 Jan 2013
Micalessi M I, Boulet G A, Vorsters A, De Wit K, Jannes G, Mijs W, Ieven M, Van Damme P, Bogers J J
Abstract excerpt
The SPF10 PCR targets a conserved 65bp region of the HPV L1 gene for broad-spectrum amplification. The LiPA assay allows subsequent genotyping of the HPV amplicons. This study aims to develop a SPF10 real-time PCR to achieve simultaneous amplification and detection of the HPV target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. The real-time PCR shows an...
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