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Article

Droplet digital PCR-based EGFR mutation detection with an internal quality control index to determine the quality of DNA.

Scientific reports - 11 Jan 2018

Kim Sung-Su, Choi Hyun-Jeung, Kim Jin Ju, Kim M Sun, Lee In-Seon, Byun Bohyun, Jia Lina, Oh Myung Ryurl, Moon Youngho, Park Sarah, Choi Joon-Seok, Chae Seoung Wan, Nam Byung-Ho, Kim Jin-Soo, Kim Jihun, Min Byung Soh, Lee Jae Seok, Won Jae-Kyung, Cho Soo Youn, Choi Yoon-La, Shin Young Kee

Abstract excerpt

In clinical translational research and molecular in vitro diagnostics, a major challenge in the detection of genetic mutations is overcoming artefactual results caused by the low-quality of formalin-fixed paraffin-embedded tissue (FFPET)-derived DNA (FFPET-DNA). Here, we propose the use of an 'internal quality control (iQC) index' as a criterion for judging the minimum quality of DNA for PCR-based analyses. In a...

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Identifiers

PMID: 29323170
DOI: 10.1038/s41598-017-18642-x
PMCID: PMC5765147

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EGFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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