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Article

Integrated sequencing of exome and mRNA of large-sized single cells.

Scientific reports - 10 Jan 2018

Wang Lily Yan, Guo Jiajie, Cao Wei, Zhang Meng, He Jiankui, Li Zhoufang

Abstract excerpt

Current approaches of single cell DNA-RNA integrated sequencing are difficult to call SNPs, because a large amount of DNA and RNA is lost during DNA-RNA separation. Here, we performed simultaneous single-cell exome and transcriptome sequencing on individual mouse oocytes. Using microinjection, we kept the nuclei intact to avoid DNA loss, while retaining the cytoplasm inside the cell membrane, to maximize the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29321653
DOI: 10.1038/s41598-017-18730-y
PMCID: PMC5762704

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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