Article
A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron.
Chemistry (Weinheim an der Bergstrasse, Germany) - 6 Apr 2018
Hunter Thérèse, Bonetta Rosalin, Sacco Anthony, Vella Marita, Sultana Paul-Michael, Trinh Chi H, Fadia Hava B R, Borowski Tomasz, Garcia-Fandiño Rebeca, Stockner Thomas, Hunter Gary J
Abstract excerpt
We have generated a site-directed mutant of the manganese superoxide dismutase SOD-3 of C.elegans (MnSOD-3) which modifies the metal specificity of the enzyme. While wild-type MnSOD-3 functions with manganese in the active site (3600 U mg-1 of protein) it has little or no activity when iron is incorporated. However, when histidine replaces glutamine 142 in the active site, the enzyme retains 50 % of its activity...
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