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Article

Evidence regarding the hypothesis that the histidine-histidine contact pairs may affect protein stability.

International journal of biological macromolecules - 1 May 2012

Haghani Karimeh, Khajeh Khosro, Naderi-Manesh Hossein, Ranjbar Bijan

Abstract excerpt

It has been lately proposed that the interaction between like-charged residues stabilizes the native state of proteins. To explore this, we created a histidine-histidine pair in the Ca-III binding site of the Bacillus amyloliquefaciens α-amylase (BAA) and then examined the impact of this pairing on the BAA. For this purpose, we used site-directed mutagenesis (SDM) to substitute Pro407 with His, Ala, Gln, Arg, and...

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Identifiers

PMID: 22257446
DOI: 10.1016/j.ijbiomac.2011.12.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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