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Engineering Clostridium absonum 7α-hydroxysteroid Dehydrogenase for Enhancing Thermostability Based on Flexible Site and ΔΔG Prediction.

Protein and peptide letters - 1 Jan 2018

Lou Deshuai, Tan Jun, Zhu Liancai, Ji Shunlin, Tang Shijin, Yao Kaiyi, Han Jingxuan, Wang Bochu

Abstract excerpt

BACKGROUND: Enhancing thermostability of the 7α-Hydroxysteroid dehydrogenases (7α-HSDHs) is beneficial to its industrial application broadly. For protein engineering to enhance thermostability the nonrational strategy, directed evolution, has been applied in obtaining more stable proteins through error-prone PCR or DNA rearrangement generating random mutations. However, the successful application of directed...

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PMID: 29141528
DOI: 10.2174/0929866524666171113113100

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MOS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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