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Article

Extreme makeover: Engineering the activity of a thermostable alcohol dehydrogenase (AdhD) from Pyrococcus furiosus.

Biotechnology journal - 1 Dec 2016

Solanki Kusum, Abdallah Walaa, Banta Scott

Abstract excerpt

Alcohol dehydrogenase D (AdhD) is a monomeric thermostable alcohol dehydrogenase from the aldo-keto reductase (AKR) superfamily of proteins. We have been exploring various strategies of engineering the activity of AdhD so that it could be employed in future biotechnology applications. Driven by insights made in other AKRs, we have made mutations in the cofactor-binding pocket of the enzyme and broadened its...

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Identifiers

PMID: 27593979
DOI: 10.1002/biot.201600152

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