Article
Altering Residue 134 Confers an Increased Substrate Range of Alkylated Nucleosides to the E. coli OGT Protein.
Molecules (Basel, Switzerland) - 11 Nov 2017
Schoonhoven Nadia M, O'Flaherty Derek K, McManus Francis P, Sacre Lauralicia, Noronha Anne M, Kornblatt M Judith, Wilds Christopher J
Abstract excerpt
O⁶-Alkylguanine-DNA alkyltransferases (AGTs) are proteins responsible for the removal of mutagenic alkyl adducts at the O⁶-atom of guanine and O⁴-atom of thymine. In the current study we set out to understand the role of the Ser134 residue in the Escherichia coli AGT variant OGT on substrate discrimination. The S134P mutation in OGT increased the ability of the protein to repair both O⁶-adducts of guanine and...
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