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Increased virulence of a PB2/HA mutant of an avian H9N2 influenza strain after three passages in porcine differentiated airway epithelial cells.

Veterinary microbiology - 1 Nov 2017

Yang Wei, Lambertz Ruth L O, Punyadarsaniya Darsaniya, Leist Sarah R, Stech Jürgen, Schughart Klaus, Herrler Georg, Wu Nai-Huei, Meng Fandan

Abstract excerpt

We analyzed the adaptation of influenza viruses to growth in differentiated airway epithelial cells of a new host by passaging an avian H9N2 virus three times in porcine precision-cut lung slices (PCLS). Sequence analysis revealed four mutations: one each in the PB2 and NS1 proteins, and two in the HA protein. In this study, we characterized the PB2 mutation G685R by generating recombinant H9N2 viruses containing...

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PMID: 29102108
DOI: 10.1016/j.vetmic.2017.10.015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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