Article
Site-specific identification and quantitation of endogenous SUMO modifications under native conditions.
Nature communications - 27 Oct 2017
Lumpkin Ryan J, Gu Hongbo, Zhu Yiying, Leonard Marilyn, Ahmad Alla S, Clauser Karl R, Meyer Jesse G, Bennett Eric J, Komives Elizabeth A
Abstract excerpt
Small ubiquitin-like modifier (SUMO) modification regulates numerous cellular processes. Unlike ubiquitin, detection of endogenous SUMOylated proteins is limited by the lack of naturally occurring protease sites in the C-terminal tail of SUMO proteins. Proteome-wide detection of SUMOylation sites on target proteins typically requires ectopic expression of mutant SUMOs with introduced tryptic sites. Here, we...
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