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Article

In Planta Processing of the SpCas9-gRNA Complex.

Plant & cell physiology - 1 Nov 2017

Mikami Masafumi, Toki Seiichi, Endo Masaki

Abstract excerpt

In CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9)-mediated genome editing in plants, Streptococcus pyogenes Cas9 (SpCas9) protein and the required guide RNA (gRNA) are, in most cases, expressed from a stably integrated transgene. Generally, SpCas9 protein is expressed from an RNA polymerase (pol) II promoter, while gRNA is expressed from a pol III promoter....

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Identifiers

PMID: 29040704
DOI: 10.1093/pcp/pcx154
PMCID: PMC5921533

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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