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Article

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues.

Journal of visualized experiments : JoVE - 22 Sept 2017

Li Shengbin, Mwakalundwa Gwantwa, Skinner Pamela J

Abstract excerpt

T cells are critical to many immunological processes, including detecting and eliminating virus-infected cells, preventing autoimmunity, assisting in B-cell and plasma-cell production of antibodies, and detecting and eliminating cancer cells. The development of MHC-tetramer staining of antigen-specific T cells analyzed by flow cytometry has revolutionized our ability to study and understand the immunobiology of T...

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Share to CD8-Positive T-Lymphocytes
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28994787
DOI: 10.3791/56130
PMCID: PMC5752327

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