Article
Limits of variation, specific infectivity, and genome packaging of massively recoded poliovirus genomes.
Proceedings of the National Academy of Sciences of the United States of America - 10 Oct 2017
Song Yutong, Gorbatsevych Oleksandr, Liu Ying, Mugavero JoAnn, Shen Sam H, Ward Charles B, Asare Emmanuel, Jiang Ping, Paul Aniko V, Mueller Steffen, Wimmer Eckard
Abstract excerpt
Computer design and chemical synthesis generated viable variants of poliovirus type 1 (PV1), whose ORF (6,189 nucleotides) carried up to 1,297 "Max" mutations (excess of overrepresented synonymous codon pairs) or up to 2,104 "SD" mutations (randomly scrambled synonymous codons). "Min" variants (excess of underrepresented synonymous codon pairs) are nonviable except for P2Min, a variant temperature-sensitive at 33...
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