Article
A Genetic Screen for Impaired Systemic RNAi Highlights the Crucial Role of DICER-LIKE 2.
Plant physiology - 1 Nov 2017
Taochy Christelle, Gursanscky Nial R, Cao Jiangling, Fletcher Stephen J, Dressel Uwe, Mitter Neena, Tucker Matthew R, Koltunow Anna M G, Bowman John L, Vaucheret Hervé, Carroll Bernard J
Abstract excerpt
Posttranscriptional gene silencing (PTGS) of transgenes involves abundant 21-nucleotide small interfering RNAs (siRNAs) and low-abundance 22-nucleotide siRNAs produced from double-stranded RNA (dsRNA) by DCL4 and DCL2, respectively. However, DCL2 facilitates the recruitment of RNA-DEPENDENT RNA POLYMERASE 6 (RDR6) to ARGONAUTE 1-derived cleavage products, resulting in more efficient amplification of secondary and...
Topics
- Arabidopsis
- Arabidopsis Proteins
- Cell Cycle Proteins
- Genes, Reporter
- Genetic Testing
- Glucuronidase
- Green Fluorescent Proteins
- Models, Biological
- Mutation
- Phenotype