Article
Doubled Haploid Laboratory Protocol for Wheat Using Wheat-Maize Wide Hybridization.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Santra Meenakshi, Wang Hong, Seifert Scott, Haley Scott
Abstract excerpt
In traditional wheat breeding, the uniformity of lines derived from a breeding population is obtained by repeated selfing from the F1 which takes several generations to reach homozygosity in loci controlling traits of interest. Using doubled haploid technology, however, it is possible to attain 100% homozygosity at all loci in a single generation and completely homogeneous breeding lines can be obtained in...
Topics
- Haploidy
- Hybridization, Genetic
- Phenotype
- Plant Breeding
- Pollination
- Seeds
- Triticum
- Zea mays
