Article
Generation of doubled haploid transgenic wheat lines by microspore transformation.
PloS one - 1 Jan 2013
Brew-Appiah Rhoda A T, Ankrah Nii, Liu Weiguo, Konzak Calvin F, von Wettstein Diter, Rustgi Sachin
Abstract excerpt
Microspores can be induced to develop homozygous doubled haploid plants in a single generation. In the present experiments androgenic microspores of wheat have been genetically transformed and developed into mature homozygous transgenic plants. Two different transformation techniques were investigated, one employing electroporation and the other co-cultivation with Agrobacterium tumefaciens. Different tissue...
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