Article
Agrobacterium-Mediated Transformation of Wheat Using Immature Embryos.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Hensel Goetz, Marthe Cornelia, Kumlehn Jochen
Abstract excerpt
Methods of the Agrobacterium-mediated transformation of bread wheat (Triticum aestivum L.) have been improved in recent years so that genetic engineering can be routinely used for functional genomics as well as for wheat breeding. In the protocol described here, immature embryos of the spring-type model genotype Bobwhite SH 98 26 have been used. Preculture and temperature pretreatment of these explants have led...
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