Article
Use of Microspore-Derived Calli as Explants for Biolistic Transformation of Common Wheat.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020
Rustgi Sachin, Kashyap Samneet, Ankrah Nii, von Wettstein Diter
Abstract excerpt
There are specific advantages of using microspores as explants: (1) A small number of explant donors are required to obtain the desired number of pollen embryoids for genetic transformation and (2) microspores constitute a synchronous mass of haploid cells, which are transformable by various means and convertible to doubled haploids therefore allow production of homozygous genotypes in a single generation....
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