Use of Microspore-Derived Calli as Explants for Biolistic Transformation of Common Wheat.

doi:10.1007/978-1-0716-0356-7_14

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Article

Use of Microspore-Derived Calli as Explants for Biolistic Transformation of Common Wheat.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Rustgi Sachin, Kashyap Samneet, Ankrah Nii, von Wettstein Diter

Abstract excerpt

There are specific advantages of using microspores as explants: (1) A small number of explant donors are required to obtain the desired number of pollen embryoids for genetic transformation and (2) microspores constitute a synchronous mass of haploid cells, which are transformable by various means and convertible to doubled haploids therefore allow production of homozygous genotypes in a single generation....

Topics

Biolistics
Chromosomes, Plant
Colchicine
DNA, Plant
Genotype
Gold
Plants, Genetically Modified
Ploidies
Pollen
Regeneration
Transformation, Genetic
Triticum

Source

Open in PubMed

Identifiers

PMID: 32277459
DOI: 10.1007/978-1-0716-0356-7_14

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