Article
Doubled Haploid Production in High- and Low-Response Genotypes of Rapeseed (Brassica napus) Through Isolated Microspore Culture.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Corral-Martínez Patricia, Camacho-Fernández Carolina, Mir Ricardo, Seguí-Simarro Jose M
Abstract excerpt
Rapeseed (Brassica napus) is one of the most important oilseed crops worldwide. It is also a model system to study the process of microspore embryogenesis, due to the high response of some B. napus lines, and to the refinements of the protocols. This chapter presents a protocol for the induction of haploid and DH embryos in B. napus through isolated microspore culture in two specific backgrounds widely used in DH...
Topics
- Acclimatization
- Brassica napus
- Crops, Agricultural
- Culture Media
- Diploidy
- Flow Cytometry
- Genotype
- Germination
- Haploidy
- Homozygote
- Molecular Biology
- Plant Breeding
- Ploidies
- Pollen
- Tissue Culture Techniques