Article
Rapid Gene Isolation Using MutChromSeq.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Steuernagel Burkhard, Vrána Jan, Karafiátová Miroslava, Wulff Brande B H, Doležel Jaroslav
Abstract excerpt
MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies...
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