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Article

Rapid gene isolation in barley and wheat by mutant chromosome sequencing.

Genome biology - 31 Oct 2016

Sánchez-Martín Javier, Steuernagel Burkhard, Ghosh Sreya, Herren Gerhard, Hurni Severine, Adamski Nikolai, Vrána Jan, Kubaláková Marie, Krattinger Simon G, Wicker Thomas, Doležel Jaroslav, Keller Beat, Wulff Brande B H

Abstract excerpt

Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene...

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Share to Chromosomes, Plant
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Polymorphism, Single Nucleotide

Share to Sequence Analysis, DNA

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Source

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Identifiers

PMID: 27795210
DOI: 10.1186/s13059-016-1082-1
PMCID: PMC5087116

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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