Article
Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress.
Scientific reports - 30 Aug 2017
Zhu Muzi, Fan Wudi, Cha Yaping, Yang Xiaofeng, Lai Zhicheng, Li Shuang, Wang Xiaoning
Abstract excerpt
As a nongenetic engineering technique, adaptive evolution is an effective and easy-to-operate approach to strain improvement. In this work, a commercial Thermoanaerobacterium aotearoense SCUT27/Δldh-G58 was successfully isolated via sequential batch fermentation with step-increased carbon concentrations. Mutants were isolated under selective high osmotic pressures for 58 passages. The evolved isolate rapidly...
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