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Article

Establishing PNB-qPCR for quantifying minimal ctDNA concentrations during tumour resection.

Scientific reports - 21 Aug 2017

Ehlert T, Tug S, Brahmer A, Neef V, Heid F, Werner C, Jansen-Winkeln B, Kneist W, Lang H, Gockel I, Simon P

Abstract excerpt

The analysis of blood plasma or serum as a non-invasive alternative to tissue biopsies is a much-pursued goal in cancer research. Various methods and approaches have been presented to determine a patient's tumour status, chances of survival, and response to therapy from serum or plasma samples. We established PNB-qPCR (Pooled, Nested, WT-Blocking qPCR), a highly specific nested qPCR with various modifications to...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28827745
DOI: 10.1038/s41598-017-09137-w
PMCID: PMC5566323

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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