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Corynebacterium glutamicum Chassis C1*: Building and Testing a Novel Platform Host for Synthetic Biology and Industrial Biotechnology.

ACS synthetic biology - 19 Jan 2018

Baumgart Meike, Unthan Simon, Kloß Ramona, Radek Andreas, Polen Tino, Tenhaef Niklas, Müller Moritz Fabian, Küberl Andreas, Siebert Daniel, Brühl Natalie, Marin Kay, Hans Stephan, Krämer Reinhard, Bott Michael, Kalinowski Jörn, Wiechert Wolfgang, Seibold Gerd, Frunzke Julia, Rückert Christian, Wendisch Volker F, Noack Stephan

Abstract excerpt

Targeted top-down strategies for genome reduction are considered to have a high potential for providing robust basic strains for synthetic biology and industrial biotechnology. Recently, we created a library of 26 genome-reduced strains of Corynebacterium glutamicum carrying broad deletions in single gene clusters and showing wild-type-like biological fitness. Here, we proceeded with combinatorial deletions of...

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PMID: 28803482
DOI: 10.1021/acssynbio.7b00261

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