Article
Identification of essential genes for cancer immunotherapy.
Nature - 31 Aug 2017
Patel Shashank J, Sanjana Neville E, Kishton Rigel J, Eidizadeh Arash, Vodnala Suman K, Cam Maggie, Gartner Jared J, Jia Li, Steinberg Seth M, Yamamoto Tori N, Merchant Anand S, Mehta Gautam U, Chichura Anna, Shalem Ophir, Tran Eric, Eil Robert, Sukumar Madhusudhanan, Guijarro Eva Perez, Day Chi-Ping, Robbins Paul, Feldman Steve, Merlino Glenn, Zhang Feng, Restifo Nicholas P
Abstract excerpt
Somatic gene mutations can alter the vulnerability of cancer cells to T-cell-based immunotherapies. Here we perturbed genes in human melanoma cells to mimic loss-of-function mutations involved in resistance to these therapies, by using a genome-scale CRISPR-Cas9 library that consisted of around 123,000 single-guide RNAs, and profiled genes whose loss in tumour cells impaired the effector function of CD8+ T cells....
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