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Article

Rapid Generation of Human Genetic Loss-of-Function iPSC Lines by Simultaneous Reprogramming and Gene Editing.

Stem cell reports - 12 Sept 2017

Tidball Andrew M, Dang Louis T, Glenn Trevor W, Kilbane Emma G, Klarr Daniel J, Margolis Joshua L, Uhler Michael D, Parent Jack M

Abstract excerpt

Specifically ablating genes in human induced pluripotent stem cells (iPSCs) allows for studies of gene function as well as disease mechanisms in disorders caused by loss-of-function (LOF) mutations. While techniques exist for engineering such lines, we have developed and rigorously validated a method of simultaneous iPSC reprogramming while generating CRISPR/Cas9-dependent insertions/deletions (indels). This...

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Identifiers

PMID: 28781079
DOI: 10.1016/j.stemcr.2017.07.003
PMCID: PMC5599229

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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