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Article

RAPD-PCR as a Rapid Approach for the Evaluation of Genotoxin-Induced Damage to Bacterial DNA.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Tofalo Rosanna, Corsetti Aldo

Abstract excerpt

RAPD PCR is a sensitive and reliable approach useful for the detection of DNA lesions due to environmental contaminants. In addition, this method is cost-effective, and can be performed in any laboratory having a DNA thermocycler and gel electrophoresis system. Here, we describe its application to identify genotoxin-induced DNA damage in foodborne bacteria. DNA alterations are detected through the analysis of...

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PMID: 28710766
DOI: 10.1007/978-1-4939-7187-9_18

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